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Semaglutide synthesis differs through its unique fatty acid side chain modification and extended half-life design, requiring specialized peptide coupling techniques and purification processes compared to shorter-acting GLP-1 agonists like liraglutide
Subsequent studies have shown that it can influence melatonin secretion, a key regulator of circadian rhythms and sleep-wake cycles
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Cell viability Cellular viability was evaluated by a CCK-8 proliferation assay using a Cell Counting Kit-8 (Nanjing Jiancheng Bioengineering Institute, China)
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