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Bound C1q or deposited C3, C4 or C5 was detected using specific primary antibodies from rabbit (all from Dako/Agilent, A0135, A0062, A0065, A0056) (diluted 1:5000 in PBS/T/S) and a secondary HRP-conjugated anti-rabbit IgG antibody from donkey (Cytiva Life Sciences, NA935) (diluted 1:10.000 in PBS/T/S)

Its stability in gastric environments and compatibility with diverse lab models (in vitro, ex vivo, in vivo) make it an indispensable research tool for exploring multi-tissue repair and vascular health mechanisms

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