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Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached

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(Flip the toggle if you already know your water amount.) Results Add this much bacteriostatic water 1 mL to your 10mg Tirzepatide vial each 2.5mg dose = 25 units on a U-100 insulin syringe (~4 doses per vial)

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