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This is especially true when the headache complaints are not accompanied by the typical features of migraine such as nausea, light/sound sensitivity, and exacerbation upon physical activity (Cutrer 2012)

Screening recommendations target adults with risk factors including overweight, family history, age over 40, and ethnicity with higher diabetes prevalence

For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)