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Alison Good (Scotland, UK) for her helpful review of the manuscript

There is little agreement regarding the possible effects of insulin, with either no or an inhibitory effect on glucagon secretion [18], and variable effects on somatostatin secretion in some studies [18, 19], and no effect of insulin on somatostatin secretion in other studies [20]

Nutr Rev 1981;29:385-90

Cells were cultured in glass bottom dishes (ibidi, Grfelfing), and multipoint images over 10 XY fields were acquired every 1 min using a 60 Plan Apo 1.4 numerical aperture (NA) Nikon oil objective (Nikon) and Perfect Focus 2.0 engaged on the CSU W1 Confocal Scanner Unit (Yokogawa) attached to a Nikon ECLIPSE Ti2 microscope with a Photometrics Prime 95B camera (Teledyne Photometrics)