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In the first step (RT-1), 1 g of total RNA was mixed with 0.6 L of oligo(dT) primer (500 g/mL, Promega) and RNase-free water to a final volume of 12 L

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162,163 Paradoxically, despite enhanced cystine uptake, MCU-overexpressed PDAC exhibited typical features of ferroptosis caused by cystine deprivation

Dillon JS, Tanizawa Y, Wheeler MB, Leng XH, Ligon BB, Rabin DU, Yoo-Warren H, Permutt MA, and Boyd AE, 3rd