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Vicente-Gutierrez C, Bonora N, Bobo-Jimenez V, Jimenez-Blasco D, Lopez-Fabuel I, Fernandez E, et al

The column was eluted isocratically at a flow rate of 450 L/min for 1 minute at 80% mobile-phase A (10 mM NH 4 Ac/methanol/acetic acid 95:5:0.1, by volume), followed by a linear gradient to 80% mobile-phase B (methanol/acetic acid