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To investigate LOD and LOQ of the analytes of interest, a proxy matrix (0.9% NaCl in 10 mmol/L phosphate buffer, pH 7.8) was spiked with decreasing concentrations of the standard solution of N-Hcy-Lys and low-molecular-mass thiols and subsequently subjected to all steps of the analytical procedure

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This can happen with: Gallstones : Hardened deposits that block the bile ducts