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First, start berberine at a low dose (typically 500 mg once or twice daily) and titrate slowly over 2-3 weeks while monitoring blood sugar, energy levels, and gastrointestinal symptoms

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NF-B is a key regulator of apoptosis, immunity, aging, and inflammation due to its role in the expression of proinflammatory genes, including cytokines, chemokines, and adhesion molecules.34 In the present study, the absence of elevated NF-B, TNF-, and IL-6 levels in melatonin- and glutathione-treated groups suggests potential modulation of inflammatory responses by these agents