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Recent publications have further highlighted the complexities and potential problems with translating findings from animal models to human migraine pathology [6]

At the indicated time (1, 2, 4, 6 and 22 h) an aliquot of 10 L was removed from each experimental reaction and pipetting into 100 L of 1% TFA solution to quench the reaction ( t = 0 min was determined using the reaction without enzyme)

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In addition, challenges like individual tolerability and competition from dual agonists, such as Eli Lillys tirzepatide, also demand further optimization [3537]