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All the experimental procedures were approved by the National Eye Institute Animal Care and Use Committee and the Ethical Committee of University of Modena and Reggio Emilia and by the Italian Ministero della Salute (150/2021-PR) and were performed as per guidelines of the Association for Research in Vision and Ophthalmology statement for the Use of Animals in Ophthalmic and Vision Research and in accordance with the ARRIVE guidelines

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Proteins were electrophoresed at 80 V (stacking gel) and 120 V (separating gel) using SDS-PAGE

doi: 10.2147/TCRM.S37314 83 ReznikYBertheratJBorson-ChazotFBrueTChansonPCortet-RudelliCet al