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The core direction of future research should focus on directly verifying the causal relationship between local joint ferroptosis and gut microbiota dysbiosis through germ-free OA mouse models, fecal microbiota transplantation experiments, or conditional knockout models of key genes for chondrocyte-specific ferroptosis (such as GPX4 and ACSL4)

These actions can be loosely divided along three lines: 1 ) classic ER activation involving homodimerization, translocation to the nucleus and transcription

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