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The plate was then incubated at 37 C with 5% CO 2 for 1 h

Structure and mode of action A molecule of HSP70 consists of an N-terminal nucleotide-binding domain (NBD), a substrate-binding domain (SBD), a linker region connecting the two, and variable C-terminal motifs such as EEVD (Flaherty et al., 1990) (Figure 1)