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Crystallization of Pt GSTF8 in presence of ()-catechin and GSH Purified Pt GSTF8 in 30 mM Tris, pH 8, 200 mM NaCl, 1 mM EDTA was concentrated to 12.4 mg ml 1 using an Amicon Ultra-4 device (10 kDa cut-off) (Merck Millipore) and supplemented with 1 mM GSH and 25 mM ()-catechin immediately before crystallization

Chen J, Yang L, Geng L, He J, Chen L, Sun Q, Zhao J, Wang X (2021) Inhibition of acyl-CoA synthetase long-chain family member 4 facilitates neurological recovery after stroke by regulation ferroptosis

Purification The crude peptide is then purified, often using High-Performance Liquid Chromatography (HPLC)

Garber A, Henry R, Ratner R, et al