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Pediatr Neurol 2003, 28: 24-27

Various substituent groups decorating the EPT nucleus are key to modulating the toxicity of trichothecenes and based on these substitution patterns, trichothecenes can be sub-classified into four groups designated Type AD

Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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