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Cell pellets (from 1 L insect cell culture) were thawed and suspended in 20 mM HEPES pH 7.4, 50 mM NaCl, 5 mM CaCl 2 , 2 mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Sigma Aldrich) and benzonase (Merk Millipore)

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Roberts has epilepsy and was feeling lethargic from the effects of a major seizure she had the day before, on her 42nd birthday, that resulted in an ambulance ride and treatment at a hospital emergency room