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If semaglutide only increases insulin production in the pancreas, that increase could dangerously lower blood sugar without an attendant increase in glucagon production.4 But if tirzepatide works harder to inhibit glucagon production than it does to increase insulin production, it can have the same positive effects on blood sugar as semaglutide without needing to produce as much insulin

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The silencing effect for each mutant was measured by qRT-PCR (see below) and is reported in Supplementary Table 2