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RNA Sequencing on mCherry Populations Lung metastatic derived cell line generated to express mCherry from the endogenous Gstt1 locus (above) was cultured under 2D conditions, followed by harvesting by trypsinization, washing twice in PBS, resuspension at 1 x 10 4 in 100ul PBS and injection into the tail vein of NOD.CB17-Prkdcscid/J mice (Jackson Laboratory) to establish lung metastases
Varying roles of this domain have been indicated, and according to the most recent results from Sinnema et al., the Zn finger ordinarily has a positive regulatory effect on the catalytic activity of PHD2
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