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These should encompass comprehensive biodistribution mapping, long-term in vivo tracking, clearance profiling, and assessments of organ-specific toxicities (hepatic, renal, pulmonary, and neurological), alongside immunogenicity assays [485]

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The fundamental distinction lies in the mechanism of action: GLP-1 agonists are receptor-specific molecules that directly stimulate GLP-1 receptors, while berberine exerts its metabolic effects primarily through AMPK activation and other pathways that do not involve direct GLP-1 receptor engagement