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The actions of agouti-related peptide (AgRP) neurons and pro-opiomelanocortin (POMC) neurons in the arcuate nucleus (ARC), the most renowned cell types of the hypothalamus governing homeostatic actions and inducing hunger, were previously understood to be mainly regulated by internal nutritional status or hormones such as leptin or ghrelin [45]

Electrophysiological signals were recorded using the Axopatch 700B amplifier (Molecular Devices), low-pass filtered at 25 kHz, and analysed offline on a PC with pCLAMP programs (Molecular Devices)

eating far less means you have far fewer opportunities to get the nutrients your body needs

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