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THF redissolved, add NaH (1.2-1.5eq) at 0~-10C, keep stirring at this temperature for half an hour, and distill ethyl bromoacetate 3 (1.2-1.5eq ) slowly drop into the reaction solution, remove the ice bath after the dropwise addition, and stir overnight at room temperature

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7c, d) in hTRPV1-expressing CHO K1 cells, whereas 1 M BCTC completely blocked these currents