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Key mutations in genes such as C24SMT, C5DS, and C14DM lead to the loss of ergosterol and the accumulation of other sterol precursors, which reduce the binding affinity of AmB to the parasite membrane and decrease drug sensitivity (Mukherjee et al., 2020)

Immunofluorescence staining The sample collection process was carried out as follows: Firstly, primary bladder cancer cells (PBCCs) and metastatic bladder cancer cells (MBCCs) expressing GFP were selected from bladder cancer tissue using flow cytometry

This finding suggested that a larger fraction of the available parent estrogens in COC users is converted into catechol estrogens and, especially in the case of E1, to methoxyestrogens