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The activity of the COX enzyme relies on its being in the oxidized form to be specific, tyrosine 385 must be oxidized to a radical.[10][11] It has been shown that paracetamol reduces the oxidized form of the COX enzyme, preventing it from forming pro-inflammatory chemicals.[7][12] Further research has shown that paracetamol also modulates the endogenous cannabinoid system.[13] Paracetamol is metabolized to AM404, a compound with several actions

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Cell Signaling) primary antibodies diluted in 2% BSA in PBST (PBS without calcium and magnesium plus 0.05% Tween-20)