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Atorvastatin activates RyR1 in a sequential way, whereby one statin per subunit can bind to the transmembrane region of a closed RyR1, with small structural perturbations that prime the channel for opening

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Farrell SE, Defendi GL, Miller MA, Burns MJ, Corden TE, Fernandez MC, et al

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The RNA samples (1 ng) were reverse transcribed into cDNA using the Hiscript III Reverse Transcriptase Kit (Vazyme, R223-01)

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Strains for further studies were stored in the MAST CRYOBANK system (Mast Diagnostic, United Kingdom) at 80 C

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