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Method 1: The detailed steps for synthesizing Retarutide using solid-state synthesis in the laboratory are as follows: Prepare the required reagents and instruments: including amino acids, dichloromethane, potassium carbonate, ethoxylated silica gel, acetonitrile, pyridine, N-methylmorpholine, diisopropylethyl chloride source, trifluoroacetic acid, DIPEA, Boc protected amino acids and other reagents, as well as magnetic stirrers, rotary evaporators and other instruments

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It is a chronic infectious disease caused by Mycobacterium tuberculosis , which enters the lungs and triggers an immune response that results in the formation of tuberculous nodules (Huang et al., 2022)