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The motifs found to be enriched in one of the clusters were then queried to find their relative frequency within each cluster vs randomly generated sets of background sequences using the Homer toolkit ( ATAC-Seq Gene Ontology Analyses Genomic coordinates for each DAR cluster ATAC-seq peaks were submitted to the GREAT 2.0.2 tool to determine if specific biological functions were enriched with differentially accessible regions at different times ( Supplementary Table 2B )