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Quantitative real-time polymerase chain reaction (qRTPCR) To determine mRNA expression, total RNA was extracted from each sample group using TRIzol reagent, and cDNA synthesis was performed using a Transcription First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland)

For patients taking alpha-glucosidase inhibitors, pure glucose (glucose tablets or gel) should be used rather than sucrose-containing foods

This proactive approach allows you to track your achievements and provides a clear understanding of the treatments effectiveness for you