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The proteins were precipitated by the addition of 1050 l water, 1400 l methanol and 350 l chloroform, followed by intense vortex and centrifugation (3200 g , 10 min at 4C)

De Maria F, Pedersen JZ, Caccuri AM, Antonini G, Turella P, Stella L et al

Case Presentation A 71-year-old female with no prior psychiatric history presented with sudden onset of: Severe anxiety Insomnia Cognitive dysfunction Fatigue Severe neck and shoulder pain Abdominal pain New intolerance to previously well-tolerated foods Symptoms began within months of moving into a new office building , suggesting a potential environmental trigger

Many people also report enhanced mood, better sleep quality, and improved cognitive performance after treatment