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However, no controlled conditions (placebo effects, natural healing, concurrent treatments confound results)

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Flag-HMGB1 or Flag-GSTP (WT), GSTP (S184A), and GSTP (S184D) were transfected into HEK293 cells, and then, the lysates were incubated with His-GSTP or His-HMGB1 immobilized on Ni 2+ IDAagarose beads (Novagen) in the lysis buffer at 4C for 8 h