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For lysine, the different forms of the amino acid would look like this: at extremely low pH, the carboxylic acid and both amine groups are protonated to give a species with a net charge of +2 At intermediate pH, the carboxylic acid is deprotonated to give a species with a net charge of +1 At basic pH, the most acidic ammonium will be deprotonated to give the neutral species with a (net) charge of 0 At strongly basic pH, both ammoniums will be deprotonated to give a species with a net charge of -1

The combination of low insulin + AOD-9604's lipolytic activation + exercise-induced fat oxidation creates optimal conditions