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The STEP 4 trial demonstrated that continuous weekly dosing was necessary to maintain weight loss, and discontinuation resulted in weight regain

Sperm viability was quantified by mixing a 2L aliquot of sperm suspension with 5L of a 1:50 dilution of SYBR-14, followed by 2L of propidium iodide (Invitrogen, L-7011, Thermo Fisher Scientific, Melbourne, Australia)