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in contrast, subclinical deficiency is common and may present with hypophosphatemia, hypocalcemia, secondary hyperparathyroidism, bone demineralization, and an increased risk of fractures

As appropriate to individual experiments, a phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, and/or a nuclear factor-B (NF-B) inhibitor, BAY11-7082, were added 1 h prior to IGF-1 treatment