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Ki67 staining The immunohistochemistry of Ki67 was conducted using mouse anti-Ki67 antibodies (Thermo Fisher, Shanghai, PA5-114437) and HRP-labeled goat anti-mouse antibodies (Servicebio, Wuhan, G23301) along with DAB chromogenic agent kit for histochemistry (Servicebio, Wuhan, GB12224)

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Newer, more specific PET tracers, such as those targeting cyclooxygenase enzymes (COX-1/2), monoamine oxidase B (MAO-B), the purinergic P2X7 receptor, colony stimulating factor-1 (CSF-1), 7 nicotinic receptors and cannabinoid 2 receptors and phosphodiesterase 4B (PDE4B) should allow for more precise characterization of neuroimmune signaling [350,351,352]

Phase 3: Maintenance Phase and Stabilizing Weight The final phase is the maintenance phase , lasting at least 3 weeks