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Severing the vagus nerve, rather than removing the appendix, can also hinder the propagation of alpha-synuclein from the gut to the brain in mice (Kim et al., 2019) and humans (Svensson et al., 2015) and improve a Parkinsons-like affliction and Parkinsons itself in, respectively, animals and humans (Liddle, 2018

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Finally, utilize the solution within 21 to 28 days to ensure optimal experimental reproducibility

To investigate the functional relevance of MOR expressed in other regions projecting to DMV Ach neurons, we bilaterally implanted cannulas into the S1, IC, PAG, PBP, and LC, respectively