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Glutathione (GSH) activity in hippocampal tissue was determined by the method described by Tipple and Rodgers [36], as follows: This colorimetric assay is based on the reaction of free thiol (-SH) groups in GSH with 5,5 -dithiobis-(2-nitrobenzoic acid) (DTNB), yielding yellow 5-thio-2-nitrobenzoic acid (TNB), measurable at 412 nm

Experimental Results: Bone mineral density (BMD): OVX control group: Lumbar BMD decreased by 28% and hip BMD decreased by 25% compared to Sham group (successful osteoporosis model)

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Peak antioxidant effects show up around 36 months of daily use