Chloroplast functionality has a positive effect on nitric oxide level in soybean cotyledons
Proline concentration was calculated from a standard curve (020 g/mL)
Among the five curated dishes are the Nutrient Power Plate (blackened chicken, roasted sweet potatoes, spicy broccoli, chickpeas, pickled onions, almonds, wild rice, golden quinoa, olive oil and lemon squeeze) and the Spicy Reset Bowl (blackened chicken, avocado, shredded cabbage, apples, chickpeas, nori sesame seasoning, wild rice, shredded kale, olive oil, hot sauce and lemon squeeze)
In brief, the PCR reaction mix contained 7.5 l KAPA SYBR FAST qPCR Master Mix (2X), 0.75 l of 5 M forward and reverse primers each, 3.5 l PCR-grade water and 2.5 l of 20-fold diluted cDNA in 15 l total reaction volume