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Murphy and George Minot for discovery of an effective treatment for pernicious anemia using liver concentrate, later found to contain a large amount of vitamin B 12

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Following overnight incubation, transformed TG1s were scraped from bioassay plates and a maxiprep was performed on scraped cultures to generate the phagemid template to replace the M13 linker with a flexible, expressible Gly-Ser-Ser-Ser-Ser or G 4 S linker