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Mitochondria, as an energy factory in eukaryotic cells, are the primary source of reactive oxygen species (ROS) in hypoxic cells and participate in the regulation of redox homeostasis
PVDF membranes were blocked with 5% skim milk at room temperature for 2 h and then incubated with primary antibodies at 4 C overnight, followed by incubation with secondary horseradish peroxidase-conjugated antibodies (1:1000, Sungene Biotech, China) for 1 h at room temperature
ACS Synth Biol 2019, 8 (2), 232238
Analyses of protein-protein interaction network by STRING database (version 12.2) among the differentially expressed proteins between PCC and uninfected individuals, showed that 19 proteins formed a single network, whereas eleven differentially expressed proteins were not related ( Fig
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