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Journal of Dental Anesthesia and Pain Medicine, 22 (2), 97

The affinity-purified shelterin was then subjected to multiple rounds of SEC, using a Superose 6 Increase 10/300 column equilibrated with the SEC buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP) to further purify and polish the complex