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Equal amounts of protein lysates were loaded on Novex WedgeWell Tris-glycine or Bis-Tris gels (ThermoFisher) and blotted onto nitrocellulose or PVDF Trans-Blot Turbo membrane (0.2 m, Bio-Rad) according to standard protocols

Other medications including: Environmental toxins Toxins in the environment like pollutants and pesticides can lead to increased intestinal permeability: Pollutants like pesticides, heavy metals, and industrial-grade chemicals can lead to toxicity in the gastrointestinal tract, damaging the intestinal lining and causing a leaky gut

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