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PMID: 26687901

Then, 1 ml of glutathione Sepharose 4B (Amersham) slurry and 1 ml of tryptophan-induced culture broth were added to 40 ml of the PBS buffer in a 50 ml tube, and kept at the room temperature for 30 min, centrifuged at 1,000 g for 10 min at the room temperature, then resuspended in the PBS buffer, and centrifuged at 1,000 g for three more times

Here are 5 benefits of glutathione: Slows the aging process Fights free radicals Reduces inflammation and cellular damage Assists with memory and mental clarity May help with autoimmune & insulin disorders Glutathione levels can naturally decline as part of the aging process

The obtained spectra were background-corrected