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Reduced glutathione (GSH) can react with dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly

This helps in breaking down the lumps and hastens the results

doi: 10.1038/s42003-021-02587-z 185 CoulterIFrewinSKrassasGEPerrosP

On the other hand, a vitamin B12 deficiency appears to cause a loss of appetite in some people, leading to weight loss rather than weight gain ( That said, the current evidence is too weak to suggest that a vitamin B12 deficiency has any strong or specific impact on weight be it weight gain or loss