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Focusing on these main differences and cell micro-environment, we now construct a model in order to explain the different features of melanoma and its different stages of progression as seen in Fig

The vitrification process leads to mitochondrial dysfunction, characterized by a reduction in mitochondrial membrane potential (MMP), an increase in ROS, and elevated mitochondrial Ca 2+ levels (44), ultimately resulting in oocyte apoptosis and diminished developmental potential (45)