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The reaction is started by the addition of substrate, DTNB or GSSG, and a second aliquot of NADPH
That property is exactly why it is preferred for vials that will be sampled more than once over several weeks in the lab
GLS, by converting glutamine into glutamate, boosts intracellular GSH synthesis to counteract ROS-induced harm and shield cells from copper-induced toxicity (Saporito-Magri et al., 2018)
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