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Use of glipizide or other antidiabetic medications must be viewed by both the physician and patient as a treatment in addition to diet and not as a substitution or as a convenient mechanism for avoiding dietary restraint

To each well was added 2 L of HEPES buffer, 38 L of the solution of oligomer to be assayed (final concentration 304 M) and 10 L of the solution of enzyme (final concentration 20 g mL 1 ) for a total volume of 50 L