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For site directed mutagenesis, plasmid template was diluted 100-fold, mixed with 0.04 U/L Phusion DNA polymerase (Thermo), 0.2 mM dNTP's, 0.5 M forward and reverse mutagenesis primers and 1x Phusion reaction buffer (Thermo), and then placed in a thermocycler for 98C for 30 s followed by 20 cycles of 10 s at 98C, 20 s at 70C, 4.5 min at 72C with a final extension at 72C for 10 min

None of the Marketing Authorisation Holders in the UK use any clinical evidence for optimal dosage of 3 monthly treatment and base the information written in their Patient Information Leaflet upon BNF Guidelines and the Martindale Drug Reference book

At Modern Medicine, you can address these deficiencies effectively with safe, medically supervised B12 and B-Well injections tailored to your health needs

Injection risks include infection at the injection site, incorrect dosing, and use of non-sterile preparations