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Under the conditions tested, only hTrxR1 C497S,U498C and hTrx1 C35S,C73S were able to form a complex that could be isolated by gel filtration chromatography and was stable in non-reducing SDSPAGE (Fig

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Compared to fresh spinach, the folate amount declined by 38% in the frozen one, mostly due to the washing step and without any effect of the blanching step during the industrial freezing processing chain 397

Dry weight and fresh weight Plants were uprooted cautiously, followed by proper washing to remove soil and weighed