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Clinical studies in obese, insulin-resistant patients further support this effect, showing that VLCKD increased Bacteroides and decreased Firmicutes [169, 171]

They speculate that a less inflammatory, less senescence-driven environment may help steroidogenic function or support progenitor-like testosterone-producing cells

Nat Med 15:629632 Singh S, Padovani D, Leslie RA, Chiku T, Banerjee R (2009) Relative contributions of cystathionine beta-synthase and gamma-cystathionase to H 2 S biogenesis via alternative trans-sulfuration reactions

Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturers instructions, with absorbance measured at 450 nm