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Xu G, Zhao X, Fu J, Wang X

The digested peptides were reconstituted in 2% acetonitrile/0.1% TFA and 5 l of each sample was loaded onto a PepMap 100 3U 75 m 20 mm reverse-phase trap where they were desalted online before being separated on a 100 2U 50-micron 150 mm PepMap EasySpray reverse-phase column 17

These effects are typically reversible once proper B12 levels have been restored

When focusing on Selenoprotein P (SELENOP), a primary selenium transport protein we found that individuals affected with konzo as compared to their unaffected sibling yielded no significant difference in protein signal in an untargeted analysis