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The cells were cultured in Ultra-Low-Attachment plates (ULA) (Thermo Scientific) in PrimNeuS media ( 4.1.3 Mice and in vivo experiments All animal experiments were approved by The Institute of Cancer Research Animal Welfare and Ethical Review Body and performed in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986, the UK National Cancer Research Institute guidelines for the welfare of animals in cancer research and the ARRIVE (animal research: reporting in-vivo experiments) guidelines
It works particularly well for researchers on moderate-dose protocols who want multiple weeks of supply from a single vial without the larger commitment (and cost) of a 30 mg vial
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